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Cells were first stained with 50 M DCFDA or 10 M mBcl, as described above, followed by a washing step with PBS and a surface staining of 30 min on ice with combinations of the following monoclonal antibodies: PE-conjugated anti-CD10 (HI10), PerCP-conjugated anti-CD3 (SK7), APC-conjugated anti-CD19 (SJ25C1), PE-conjugated anti-CD5 (L17F12), PE-conjugated anti-CD25 (PC61), all from BD Biosciences
Mitigation: Always dose post-workout (your muscle uptake of glucose is highest then blood glucose crash is less severe) Have fast-acting carbs nearby for the first several doses (fruit, dextrose) Never inject fasted Start at 20 mcg and assess blood glucose response before titrating Consider running a continuous glucose monitor (CGM) for the first cycle Libre or Dexcom gives you real-time data If you're already running GLP-1s (Reta, Tirz, semaglutide), add IGF-1 LR3 cautiously the appetite suppression from GLP-1s means you may not feel hunger signals that would otherwise warn you about low blood glucose
ERJ Open Res
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